Interview about our Corona Virus Research
Corona: Mit High-Tech gegen das Virus. - Link - zur Webseite des Bayerischen Rundfunks.
- Link - zum Bericht in der Abenschau vom 23.2.2021
Regulation and drugability of chromatin remodeling enzymes
Sequence and functional differences in the ATPase domains of CHD3 and SNF2H promise potential for selective regulability and drugability. Helen Hoffmeister et al. FEBS J. 2021, doi: 10.1111/febs.15699
Chromatin remodelers use the energy of ATP hydrolysis to regulate chromatin dynamics. Their impact for development and disease requires strict enzymatic control. Here, we address the differential regulability...
We are member of the new Transregio 305. "Über die Analyse der metastatischen Koloniebildung zu neuen systemischen Krebstherapien"
Targeting Chromatin Remodeling to inhibit Tumor Metastasis
Chromatin remodeling complexes are ATP-dependent enzymes that regulate all DNA dependent processes by moving, exchanging orevicting nucleosomes from the underlying DNA. These epigenetic modifiers play important roles in cellular homeostasis and are preferentially mutated in many diseases and cancer. Baz2a is aremodeling protein that is targeted...
The Bayerische Forschungsstiftung supports our Corona Research. "Inhibiting SARS-CoV-2 N-Protein-mediated infectivity"
Die Verpackung des SARS-CoV-2 Genoms in das Viruspartikel ist ein zentraler Schritt derVirusvermehrung, der durch das virale N-Protein reguliert wird. Im Verbund von Biochemikern,Zellbiologen, Drug-Discovery-Experten und Virologen werden wir Inhibitoren der N-Protein-Funktionidentifizieren. Die Unterbindung der Genomverpackung hat das Ziel, die Bildung infektiöser Viren zuunterbinden und den viralen Lebenszyklus...
Time resolved changes of Adenovirus chromatin structure
A pVII positioning code determines the dynamics of Adenoviral nucleoprotein structure and primes it for early gene activation Uwe Schwartz et al., (2020) BioRxiv; ID: https://doi.org/10.1101/2020.07.07.190926
Inside the capsid adenovirus DNA is associated with the structural protein pVII. However, the viral DNA organisation, pVII positioning and the dynamic changes of viral packaging upon infection, to...
Triple helix structures are stablilized by nucleosomes
Nucleosomes Stabilize ssRNA-dsDNA Triple Helices in Human Cells Rodrigo Maldonado et al., (2019) Molecular Cell; PMID: 30770238
Chromatin-associated non-coding RNAs modulate the epigenetic landscape and its associated gene expression program. The formation of triple helices is one mechanism of sequence-specific targeting of RNA to chromatin. With this study, we show an important role of the nucleosome...
Transfecting eukaryotic cells with chromatin
Antibody-targeted chromatin enables effective intracellular delivery and functionality of CRISPR/Cas9 expression plasmids Killian et al., (2019) Nucleic Acids Research; PMID: 30809660
We report a novel system for efficient and specific targeted delivery of large nucleic acids to and into cells. Plasmid DNA and core histones were assembled to chromatin by salt gradient dialysis and subsequently connected...
Functional characterisation of the human chromatin remodeling enzyme BRG1
Elucidation of the functional role of the Q- and I-motif in the human chromatin remodeling enzyme BRG1
Helen Hoffmeister et al., (2019) JBC; PMID: 30647132
The Snf2 proteins, comprising 53 different enzymesin humans, belong to the SF2 family. Many Snf2enzymes possess chromatin remodeling activity,requiring a functional ATPase domain consisting ofconserved motifs named Q and I-VII. These motifsform...
The folding into regular higher order structures of chromatin may not exist
Characterizing the nuclease accessibility of DNA in human cells to map higher order structures of chromatin.
Uwe Schwartz et al., (2018) Nucleic Acids Research; PMID: 30496478
Packaging of DNA into chromatin regulates DNA ac-cessibility and consequently all DNA-dependent pro-cesses. The nucleosome is the basic packaging unitof DNA forming arrays that are suggested, by bio-chemical studies, to fold...
Purine– and pyrimidine–triple-helix-forming oligonucleotides recognize qualitatively different target sites
Rodrigo Maldonado et al., (2017) RNA; PMID: 29222118
Triplexes are noncanonical DNA structures, which are functionally associated with regulation of gene expression through ncRNAtargeting to chromatin. Based on the rules of Hoogsteen base-pairing, polypurine sequences of a duplex can potentially formtriplex structures with single-stranded oligonucleotides. Prediction of triplex-forming sequences by bioinformatics...
